- Expression of specific microRNAs in tissue and plasma in colorectal cancer
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Allan Fellizar, Vivencio Refuerzo, John Donnie Ramos, Pia Marie Albano
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J Pathol Transl Med. 2023;57(3):147-157. Published online May 3, 2022
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DOI: https://doi.org/10.4132/jptm.2022.02.19
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 Abstract
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- Background
MicroRNAs (miRNA/miR) play significant roles in the regulation of cell differentiation, cell cycle progression, and apoptosis. They become dysregulated during carcinogenesis and are eventually released into the circulation, enabling their detection in body fluids. Thus, this study compared the miRNA expression in tissue and plasma samples of colorectal cancer (CRC) patients and clinically healthy controls and determined miRNA expression as a potential CRC biomarker.
Methods
Using quantitative reverse transcription polymerase chain reaction (RT-qPCR), miR-21-5p, miR-29a-3p, miR-92a-3p, miR-135b-5p, miR-196b-5p, and miR-197-3p, expression was analyzed and compared between the malignant (n = 41) and the adjacent neoplasm free mucosal tissues (n = 41) of CRC patients. The findings were validated in plasma samples (n = 36) collected from the same CRC patients prior to surgery or any form of treatment and compared to plasma from their age and sex-matched controls (n = 36).
Results
MiR-21-5p, miR-29a-3p, miR-92a-3p, and miR- 196b-5p were upregulated and miR-135b-5p was downregulated in CRC malignant tissues compared to their expression in adjacent neoplasm-free tissue. This was further observed in the plasma of the same CRC cases compared to controls. MiR-92a-3p showed itself the most sensitive (0.93; p < .001) and most specific (0.95; p < .001) in detecting CRC in tissue. In plasma, miR-196b-5p was the most sensitive (0.97; p < .001) and specific (0.94; p < .001) in detecting CRC. Plasma miR-92a-3p and miR-196b-5p were the most sensitive (0.95; p < .001) and specific (0.94; p < .001) in the early detection of CRC.
Conclusions
Results show that specific miRNAs dysregulated in malignant
tissues are released and can be detected in the circulation, supporting their potential as non-invasive biomarkers of CRC.
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Citations
Citations to this article as recorded by  - The Role of Extracellular Vesicles in Colorectal Cancer
Yujian Xia, Chaoran Yu, Ernest Johann Helwig, Yousheng Li
Technology in Cancer Research & Treatment.2023;[Epub] CrossRef - HNRNPA2B1-Mediated MicroRNA-92a Upregulation and Section Acts as a Promising Noninvasive Diagnostic Biomarker in Colorectal Cancer
Yiling Li, Kexin Li, Xiaoying Lou, Yue Wu, Samuel Seery, Danfei Xu, Yuqing Pei, Benheng Qian, Yuxin Wu, Shuang Liang, Kui Wu, Wei Cui
Cancers.2023; 15(4): 1367. CrossRef - Role of salivary miRNAs in the diagnosis of gastrointestinal disorders: a mini-review of available evidence
Maria Oana Săsăran, Claudia Bănescu
Frontiers in Genetics.2023;[Epub] CrossRef - Exploring the Role of Circulating Cell-Free RNA in the Development of Colorectal Cancer
Chau-Ming Kan, Xiao Meng Pei, Martin Ho Yin Yeung, Nana Jin, Simon Siu Man Ng, Hin Fung Tsang, William Chi Shing Cho, Aldrin Kay-Yuen Yim, Allen Chi-Shing Yu, Sze Chuen Cesar Wong
International Journal of Molecular Sciences.2023; 24(13): 11026. CrossRef - Lynch Syndrome Biopathology and Treatment: The Potential Role of microRNAs in Clinical Practice
Serena Ascrizzi, Grazia Maria Arillotta, Katia Grillone, Giulio Caridà, Stefania Signorelli, Asad Ali, Caterina Romeo, Pierfrancesco Tassone, Pierosandro Tagliaferri
Cancers.2023; 15(15): 3930. CrossRef
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